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goat anti serum against wheat germ agglutinin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories goat anti serum against wheat germ agglutinin
    Goat Anti Serum Against Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+serum+against+wheat+germ+agglutinin/Anti-Wheat+Germ+Agglutinin+(WGA)%2C+Unconjugated/pmc06608990-104-27-36
    Average 93 stars, based on 131 article reviews
    goat anti serum against wheat germ agglutinin - by Bioz Stars, 2026-10
    93/100 stars

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    Blocking Assay:

    Article Title: Murine Arcuate Nucleus Kisspeptin Neurons Communicate with GnRH Neurons In Utero
    Article Snippet: In the case of using two primary antibodies from rabbit, sections were first stained with either rabbit antibody to ERα, to GnRH, or to Kisspeptin, followed by monovalent Cy3-donkey antibody to rabbit IgG (1:500; catalog #711-487-003, The Jackson Laboratory) for 2 h at 20–25°C, and then blocked with 1% ChromPure rabbit IgG (catalog #011-000-008, The Jackson Laboratory) for 2 h followed by Alexa Fluor 488 rabbit antibody to GFP (1:1000; Invitrogen) for 2 h at 20–25°C. .. To visualize the transneuronal tracers BL and GFP-TTC, 14 μm sagittal embryo head and coronal P2 brain sections from KissIC/R26-BIZ, KissIC/R26-GTT, and KissIC/R26GTT/R26BIZ mice were treated with goat anti-serum against wheat germ agglutinin [1:1000; catalog #AS-2024, Vector Laboratories (which recognizes BL)] in TNB blocking solution at 4°C followed by biotinylated horse anti-goat IgG (catalog #BA-9500, Vector Laboratories) at 1:500 for 1 h at 20–25°C or with rabbit anti-GFP (catalog #A11122, Invitrogen) at 1:15,000 in TNB blocking solution at 4°C overnight followed by biotinylated goat antiserum against rabbit IgG (1:5000; catalog #BA-1000, Vector Labs) for 1 h at 20–25°C, and then treated with components of the TSA kit (BL; PerkinElmer) or the TSA+ kit (GFP; PerkinElmer) according to the manufacturer's instruction. .. Sections were then incubated with Alexa Fluor 546-streptavidin (1:500; catalog # {"type":"entrez-protein","attrs":{"text":"S11225","term_id":"103997","term_text":"pir||S11225"}} S11225 , Invitrogen) in TNB blocking solution for 30 min at 20–25°C followed by rabbit anti-GnRH (1:1000) at 4°C overnight and Dylight488-donkey antiserum against rabbit IgG (catalog #SA5-10038, Thermo Scientific) for 2 h at 20–25°C.

    Article Title: Murine Arcuate Nucleus Kisspeptin Neurons Communicate with GnRH Neurons In Utero
    Article Snippet: In the case of using two primary antibodies from rabbit, sections were first stained with either rabbit antibody to ER , to GnRH, or to Kisspeptin, followed by monovalent Cy3-donkey antibody to rabbit IgG (1:500; catalog #711-487-003, The Jackson Laboratory) for 2 h at 20 –25°C, and then blocked with 1% ChromPure rabbit IgG (catalog #011-000-008, The Jackson Laboratory) for 2 h followed by Alexa Fluor 488 rabbit antibody to GFP (1:1000; Invitrogen) for 2 h at 20 –25°C. .. To visualize the transneuronal tracers BL and GFP-TTC, 14 m sagittal embryo head and coronal P2 brain sections from KissIC/R26-BIZ, KissIC/R26-GTT, and KissIC/R26GTT/R26BIZ mice were treated with goat anti-serum against wheat germ agglutinin [1:1000; catalog #AS-2024, Vector Laboratories (which recognizes BL)] in TNB blocking solution at 4°C followed by biotinylated horse anti-goat IgG (catalog #BA-9500, Vector Laboratories) at 1:500 for 1 h at 20 – 25°C or with rabbit anti-GFP (catalog #A11122, Invitrogen) at 1:15,000 in TNB blocking solution at 4°C overnight followed by biotinylated goat antiserum against rabbit IgG (1:5000; catalog #BA-1000, Vector Labs) for 1 h at 20 –25°C, and then treated with components of the TSA kit (BL; PerkinElmer) or the TSA kit (GFP; PerkinElmer) according to the manufacturer’s instruction. .. Sections were then incubated with Alexa Fluor 546-streptavidin (1: 500; catalog #S11225, Invitrogen) in TNB blocking solution for 30 min at 20 –25°C followed by rabbit anti-GnRH (1:1000) at 4°C overnight and Dylight488-donkey antiserum against rabbit IgG (catalog #SA5-10038, Thermo Scientific) for 2 h at 20 –25°C.



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    93
    Vector Laboratories goat anti serum against wheat germ agglutinin
    Goat Anti Serum Against Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+serum+against+wheat+germ+agglutinin/Anti-Wheat+Germ+Agglutinin+(WGA)%2C+Unconjugated/pmc06608990-104-27-36
    Average 93 stars, based on 1 article reviews
    goat anti serum against wheat germ agglutinin - by Bioz Stars, 2026-10
    93/100 stars
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